Nielsen F, Nielsen K K, Brøsen K
Department of Clinical Pharmacology, Odense University, Denmark.
J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):103-10. doi: 10.1016/0378-4347(94)00259-2.
A specific and sensitive method for the quantitation of quinidine, (3S)-3-hydroxyquinidine, quinidine N-oxide, and dihydroquinidine in plasma and urine has been developed. The method is based on a single-step, liquid-liquid extraction procedure, followed by isocratic reversed-phase high-performance liquid chromatography, with fluorescence detection. After extraction from 250 microliters plasma and 100 microliters urine, the limit of determination is 10 nM and 25 nM, respectively. For the use as standards, commercially available quinidine was purified from dihydroquinidine; quinidine N-oxide was synthesized.
已开发出一种用于定量测定血浆和尿液中奎尼丁、（3S）-3-羟基奎尼丁、奎尼丁N-氧化物和二氢奎尼丁的特异且灵敏的方法。该方法基于单步液-液萃取程序，随后进行等度反相高效液相色谱法，并采用荧光检测。从250微升血浆和100微升尿液中萃取后，测定限分别为10 nM和25 nM。用作标准品的市售奎尼丁从二氢奎尼丁中纯化得到；奎尼丁N-氧化物则通过合成获得。
