Gomis D B, Escotet Arias V J, Fidalgo Alvarez L E, Gutiérrez Alvarez M D
Departamento de Química Física y Analítica, Universidad de Oviedo, Spain.
J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):49-55. doi: 10.1016/0378-4347(94)00279-7.
A reversed-phase high performance liquid chromatographic method is described for the simultaneous determination of vitamins D3, E and K1 and retinyl palmitate in plasma. Narrow-bore columns are recommended because this alternative provides a good separation efficiency, plus greater economy and sensitivity. Detection limits for individual vitamins range from 0.42 to 2.8 ng. All vitamins were separated in less than 9 min. Recovery studies showed good results for all solutes (88.8-100.3%) and the intra-day coefficients of variations ranged from 1.0 to 4.5%. This method permits the simple determination of fat-soluble vitamins using 1 ml of cattle heparinized plasma.
本文描述了一种反相高效液相色谱法，用于同时测定血浆中的维生素D3、维生素E、维生素K1和视黄醇棕榈酸酯。推荐使用窄孔柱，因为这种方法分离效率高，经济性和灵敏度也更高。各维生素的检测限在0.42至2.8纳克之间。所有维生素在9分钟内即可分离。回收率研究表明，所有溶质的回收率都很好（88.8-100.3%），日内变异系数在1.0至4.5%之间。该方法只需1毫升牛肝素化血浆，就能简单地测定脂溶性维生素。
