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[利用抗球蛋白试验检测生物体液中抗肠道病原菌抗体的研究]

[Use of the antiglobulin test for the purpose of detecting antibodies to enteropathogenic bacteria in biological fluids].

作者信息

Gennad'eva T Ia, Nikolaeva T A, Sholud'ko G S, Khazenson L B

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1976 Jan(1):109-15.

PMID:785878
Abstract

Use of IHAT with the addition of antiserum to immunoglobulins G, M, A increased the frequency of detection of antibodies to enteropathogenic bacteria in various biological fluids (blood, saliva, human milk, feces). At the same time the antiglobulin test permitted determination of the immunological class of these antibodies. In the substrates where the antibodies were subjected to an intensive action of proteolytic enzymes there was seen a decrease of the efficacy of the modified IHAT, but by results it exceeded the common IHAT. Antiglobulin test with the addition of antisera to immunoglobulins G, M and A could be recommended for the immunological study of the blood sera and of the external secretions for the purpose of the most reliable detection and determination of the immunological class of antibodies to enteropathogenic bacteria.

摘要

在免疫血凝试验(IHAT)中添加抗免疫球蛋白G、M、A血清,可提高在各种生物体液(血液、唾液、人乳、粪便)中检测肠道致病菌抗体的频率。同时,抗球蛋白试验可确定这些抗体的免疫类别。在抗体受到蛋白水解酶强烈作用的底物中,改良后的免疫血凝试验的效力有所降低,但结果仍超过普通免疫血凝试验。添加抗免疫球蛋白G、M和A血清的抗球蛋白试验,可推荐用于血清和外分泌液的免疫学研究,以便最可靠地检测和确定肠道致病菌抗体的免疫类别。

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