van Kooten T G, Schakenraad J M, van der Mei H C, Dekker A, Kirkpatrick C J, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Med Eng Phys. 1994 Nov;16(6):506-12. doi: 10.1016/1350-4533(94)90077-9.
In this study, human umbilical vein and human saphenous vein endothelial cells were seeded on glass and exposed to fluid shear in a parallel-plate flow chamber. Cell retention, morphology and migration were studied as a function of shear stress and of adhesion time prior to exposure to shear. Three-hour and 24-h adhesion times gave rise to comparable cell retention values after 2 h of flow for both cell types. Cell retention decreased from 85 to 20% as shear stress increased from 88 to 264 dynes cm-2 (8.8 to 26 Pa). Mean spreading areas decreased after the onset of flow, but subsequently stabilized to plateau values, which were smaller at higher shear stresses. Shape factors increased faster to higher values as cells were exposed to higher shear stresses, without any obvious preference in orientation of the cells with respect to the direction of flow. Migration was unidirectional with flow and linear with time. Migration was faster for cells which had adhered for 24 h than for cells which had adhered for 3 h and was accompanied by the presence of fibrillar structures left behind on the surface upstream of migrating cells. It is concluded that after 3 h adhesion to glass, cells have adhered with an adhesion strength that does not substantially increase during the next 21 h. However, during this time changes in cell-substratum interactions seem to occur judging by the differences in, e.g., migration rates.
在本研究中，将人脐静脉和人隐静脉内皮细胞接种于玻璃片上，并在平行板流动腔中使其暴露于流体剪切力下。研究了细胞保留率、形态和迁移情况，作为剪切应力以及暴露于剪切力之前的黏附时间的函数。对于这两种细胞类型，3小时和24小时的黏附时间在流动2小时后产生了相当的细胞保留率值。随着剪切应力从88达因/平方厘米（8.8帕斯卡）增加到264达因/平方厘米（26帕斯卡），细胞保留率从85%降至20%。流动开始后，平均铺展面积减小，但随后稳定在平台值，在较高剪切应力下该值较小。随着细胞暴露于更高的剪切应力，形状因子更快地增加到更高的值，细胞在流动方向上没有明显的取向偏好。迁移与流动方向一致且随时间呈线性关系。黏附24小时的细胞比黏附3小时的细胞迁移速度更快，并且在迁移细胞上游的表面上会留下纤维状结构。得出的结论是，细胞在与玻璃黏附3小时后，其黏附强度在接下来的21小时内基本没有增加。然而，从例如迁移速率的差异判断，在此期间细胞与基质的相互作用似乎发生了变化。
