酶促合成的D-葡糖醛-6-磷酸对代谢酶的选择性抑制作用
Selective inhibition of metabolic enzymes by enzymatically synthesized D-glucal-6-phosphate.
作者信息
Chenault H K, Mandes R F
机构信息
Department of Chemistry and Biochemistry, University of Delaware, Newark 19716.
出版信息
Bioorg Med Chem. 1994 Jul;2(7):627-9. doi: 10.1016/0968-0896(94)85010-0.
Yeast hexokinase (EC 2.7.1.1) catalyzes the phosphorylation of D-glucal and methyl alpha- and beta-D-glucopyranosides at 1-5% of the rates of phosphorylation of D-glucose and 2-deoxy-D-glucose. Maltose, cellobiose, D-galactal and tetrahydropyran-2-methanol are not substrates of hexokinase. Enzymatically synthesized D-glucal-6-phosphate inhibits rabbit muscle phosphoglucose isomerase competitively (KI = 1.94 mM) and phosphoglucomutase noncompetitively (KI = 0.122 mM).
酵母己糖激酶（EC 2.7.1.1）催化D-葡糖醛和α-及β-D-吡喃葡萄糖苷甲基化，其磷酸化速率为D-葡萄糖和2-脱氧-D-葡萄糖磷酸化速率的1-5%。麦芽糖、纤维二糖、D-半乳糖醛和四氢吡喃-2-甲醇不是己糖激酶的底物。酶促合成的D-葡糖醛-6-磷酸竞争性抑制兔肌肉磷酸葡萄糖异构酶（KI = 1.94 mM），非竞争性抑制磷酸葡萄糖变位酶（KI = 0.122 mM）。
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