超氧化物歧化酶-1转基因小鼠轻度局灶性脑缺血后c-fos mRNA的表达

Expression of c-fos mRNA after a mild focal cerebral ischemia in SOD-1 transgenic mice.

作者信息

Kamii H, Kinouchi H, Sharp F R, Epstein C J, Sagar S M, Chan P H

机构信息

Department of Neurosurgery, University of California at San Francisco.

出版信息

Brain Res. 1994 Oct 31;662(1-2):240-4. doi: 10.1016/0006-8993(94)90818-4.

DOI:10.1016/0006-8993(94)90818-4

PMID:7859076

Abstract

To clarify the role of oxygen free radicals in expression of the c-fos protooncogene, the distribution of c-fos mRNA was investigated in CuZn-superoxide dismutase (SOD-1) transgenic (Tg) mice compared to control nontransgenic (nTg) littermates after a mild (i.e. 10 min) focal cerebral ischemia. c-fos mRNA expression occurred at 1 to 6 h after reperfusion in the ipsilateral hippocampus and thalamus in Tg mice, whereas it did only at 1 h in the same regions in nTg mice. In the ipsilateral cortex, there were no significant differences in the pattern of the expression between nTg and Tg mice. These results suggest that oxygen radicals may suppress the expression of c-fos in the hippocampus and thalamus, the areas known to be without blood supply from the middle cerebral artery, following a mild focal cerebral ischemia and reperfusion.

摘要

为阐明氧自由基在c-fos原癌基因表达中的作用，研究了与对照非转基因（nTg）同窝小鼠相比，轻度（即10分钟）局灶性脑缺血后铜锌超氧化物歧化酶（SOD-1）转基因（Tg）小鼠中c-fos mRNA的分布情况。Tg小鼠再灌注后1至6小时，同侧海马体和丘脑中出现c-fos mRNA表达，而nTg小鼠在相同区域仅在1小时出现表达。在同侧皮质中，nTg和Tg小鼠之间的表达模式没有显著差异。这些结果表明，在轻度局灶性脑缺血和再灌注后，氧自由基可能会抑制海马体和丘脑中c-fos的表达，已知这些区域没有大脑中动脉供血。