使用新的细胞发育指数和定量干涉显微镜比较颊上皮细胞和鼻上皮细胞。
Comparison of buccal and nasal epithelial cells using a new cell development index and quantitative interference microscopy.
作者信息
Pappelis C K, Slobin J, Corvallis J, Detwiler H D, Pappelis A J, Pappelis G A
出版信息
Acta Cytol. 1976 Jul-Aug;20(4):372-4.
Cell area, cell dry mass, and three summary indices for tissue maturation were obtained for human buccal and nasal cells viewed in smears (five donors). The means for cell area and dry mass in the buccal smears were 3816 X 10(-8) cm2 and 2294 pg (1 pg=1 X 10(-12) g), respectively. The correlation coefficient for means of cell area and dry mass was r=0.03. The means for the three maturation indices used to characterize the cells viewed in buccal smears were: Cell Development Index (CDI), 486; Maturation Index (MI) 50.6; and Karyopyknotic Index (KPI), 0.028. The correlation coefficients for means were: CDI vs. MI, r=0.88; CDI vs. KPI, r=0.71; and MI vs. KPI, r=0.73. In the buccal smears, the cell types found most frequently were intermediate with round or oval nuclei, which altogether averaged about 90% of the total smear composition, while superficial cells (pyknotic ghost, and anucleate) comprised less than four per cent. In nasal smears, the means for cell area and dry mass were 867 X 10(-8) cm2 and 623 pg, respectively. The correlation coefficient for means of cell area and dry mass was r=0.74. The means for the maturation indices were: CDI, 816; MI, 91.2; and KPI, 0.79. The correlation coefficients for means were CDI vs. MI, r=0.93; CDI vs. KPI, r=0.97; and MI vs. KPI, r=0.85. In nasal smears, the anucleate cell type was found most frequently (about 75%) while intermediate cells with round or oval nuclei comprised approximately 13% of the total smear composition. When donor means for cell area and dry mass of buccal cells were combined for study, they were highly correlated (r=0.88). Similarly, when the maturation indices for buccal and nasal cells were combined for study, they were highly correlated: CDI vs. MI, r=0.99; CDI vs. KPI, r=0.99; and MI vs. KPI, r=0.99. Of the three indices, we believe that the CDI is the index best suited for use in studies of cell development because it is based on an inferred sequence of cellular and nuclear changes occurring during the maturation of epithelial cells. It provides a more detailed description of the cells observed in the smears. It also correlates highly in KPI and MI used in clinical and research efforts.
对涂片观察的人类颊细胞和鼻细胞（5名供体）获取了细胞面积、细胞干质量以及用于组织成熟的三个汇总指标。颊涂片的细胞面积和干质量均值分别为3816×10⁻⁸平方厘米和2294皮克（1皮克 = 1×10⁻¹²克）。细胞面积和干质量均值的相关系数为r = 0.03。用于表征颊涂片观察细胞的三个成熟指标的均值为：细胞发育指数（CDI）486；成熟指数（MI）50.6；核固缩指数（KPI）0.028。均值的相关系数为：CDI与MI，r = 0.88；CDI与KPI，r = 0.71；MI与KPI，r = 0.73。在颊涂片中，最常见的细胞类型是核呈圆形或椭圆形的中间细胞，它们总计约占涂片总成分的90%，而表层细胞（固缩的幽灵细胞和无核细胞）占比不到4%。在鼻涂片中，细胞面积和干质量均值分别为867×10⁻⁸平方厘米和623皮克。细胞面积和干质量均值的相关系数为r = 0.74。成熟指标的均值为：CDI 816；MI 91.2；KPI 0.79。均值的相关系数为：CDI与MI，r = 0.93；CDI与KPI，r = 0.97；MI与KPI，r = 0.85。在鼻涂片中，最常见的细胞类型是无核细胞（约75%），而核呈圆形或椭圆形的中间细胞约占涂片总成分的13%。当将颊细胞的供体细胞面积和干质量均值合并进行研究时，它们高度相关（r = 0.88）。同样，当将颊细胞和鼻细胞的成熟指标合并进行研究时，它们高度相关：CDI与MI，r = 0.99；CDI与KPI，r = 0.99；MI与KPI，r = 0.99。在这三个指标中，我们认为CDI是最适合用于细胞发育研究的指标，因为它基于上皮细胞成熟过程中发生的细胞和核变化的推断序列。它对涂片中观察到的细胞提供了更详细的描述。它在临床和研究工作中使用的KPI和MI方面也高度相关。
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