Squarzoni S, Cinti C, Santi S, Valmori A, Maraldi N M
Istituto di Citomorfologia Normale e Patologica CNR, Bologna, Italy.
Chromosoma. 1994 Oct;103(6):381-92. doi: 10.1007/BF00362282.
In the past, ultrastructural studies on chromosome morphology have been carried out using light microscopy, scanning electron microscopy and transmission electron microscopy of whole mounted or sectioned samples. Until now, however, it has not been possible to use all of these techniques on the same specimen. In this paper we describe a specimen preparation method that allows one to study the same chromosomes by transmission, scanning-transmission and scanning electron microscopy, as well as by standard light microscopy and confocal microscopy. Chromosome plates are obtained on a carbon coated glass slide. The carbon film carrying the chromosomes is then transferred to electron microscopy grids, subjected to various treatments and observed. The results show a consistent morphological correspondence between the different methods. This method could be very useful and important because it makes possible a direct comparison between the various techniques used in chromosome studies such as banding, in situ hybridization, fluorescent probe localization, ultrastructural analysis, and colloidal gold cytochemical reactions.
过去，人们利用光学显微镜、扫描电子显微镜和透射电子显微镜对整装或切片样本进行染色体形态的超微结构研究。然而，迄今为止，还无法在同一标本上使用所有这些技术。在本文中，我们描述了一种标本制备方法，该方法可使人们通过透射电子显微镜、扫描透射电子显微镜和扫描电子显微镜，以及标准光学显微镜和共聚焦显微镜来研究相同的染色体。在涂有碳膜的载玻片上获得染色体玻片。然后将承载染色体的碳膜转移到电子显微镜网格上，进行各种处理并观察。结果表明，不同方法之间存在一致的形态对应关系。该方法可能非常有用且重要，因为它使得在染色体研究中使用的各种技术（如显带、原位杂交、荧光探针定位、超微结构分析和胶体金细胞化学反应）之间进行直接比较成为可能。
