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[啮齿动物与食肉动物胰腺激肽释放酶之间抗原关系的免疫化学评估]

[Immunochemical assessment of the antigenic relationship between the pancreatic kallikrein of rodents and carnivores].

作者信息

Protsenko V A, Kosik O G, Veselov V F

出版信息

Zh Evol Biokhim Fiziol. 1978 May-Jun;14(3):298-300.

PMID:78602
Abstract

Using quantitative and qualitative immunochemical methods, in combination with isoelectrical focusing, it was shown that antibodies to commercial kallikrein are involved into precipitation reaction with kallikrein in extracts from the pancreas of dogs, cats, guinea pigs, rats and mice. Complexes antigen--antibody exhibit similar physico-chemical properties; during isoelectric focusing, they are found in the same pH range as the immunoactive portion of commercial kallikrein. These data indicate antigenic similarity of pancreatic kallikrein in different mammalian species.

摘要

运用定量和定性免疫化学方法,并结合等电聚焦技术,结果显示,针对商用激肽释放酶的抗体可与犬、猫、豚鼠、大鼠和小鼠胰腺提取物中的激肽释放酶发生沉淀反应。抗原 - 抗体复合物呈现出相似的物理化学性质;在等电聚焦过程中,它们所处的pH范围与商用激肽释放酶的免疫活性部分相同。这些数据表明不同哺乳动物物种的胰腺激肽释放酶具有抗原相似性。

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