Reyes J G, Bacigalupo J, Araya R, Benos D J
Instituto de Quimica, Universidad Catolica de Valparaiso, Chile.
J Reprod Fertil. 1994 Nov;102(2):313-9. doi: 10.1530/jrf.0.1020313.
The membrane potential of rat spermatids was estimated as -22 +/- 2 mV (mean +/- SEM) using three independent methods: using oxonol as a fluorescent membrane potential sensitive probe, from the passive distribution of hydrogen ions and from whole-cell patch-clamp records. The estimated permeability ratios PK+:PCl- and PNa+:PCl- of the plasma membrane of rat spermatids were 1.0 and 0.3, respectively. These data indicate that the high luminal K+ concentration found in seminiferous tubules could partially close voltage-sensitive calcium channels in these cells.
使用三种独立方法估计大鼠精子细胞的膜电位为-22±2 mV（平均值±标准误）：使用氧杂菁作为荧光膜电位敏感探针、根据氢离子的被动分布以及全细胞膜片钳记录。大鼠精子细胞质膜的渗透率比PK⁺:PCl⁻和PNa⁺:PCl⁻分别为1.0和0.3。这些数据表明，在生精小管中发现的高管腔钾离子浓度可能会部分关闭这些细胞中的电压敏感性钙通道。
