Yee H Y, Nelson J D, Jackson B
Detroit Medical Center University Laboratories, MI 48201.
J Anal Toxicol. 1994 Nov-Dec;18(7):415-8. doi: 10.1093/jat/18.7.415.
A simple method of deproteinizing whole blood with an 0.8M (5%, v/v) nitric acid solution containing Triton X-100 (0.1%, v/v) is described. The resulting supernatant is used for the measurement of lead by Zeeman graphite furnace atomic absorption spectrometry using calibration with aqueous standards. Recoveries ranged from 97.7-105.6% when an aqueous lead solution was added to a deproteinized sample supernatant and from 98.5-104.5% when whole blood control material was added to a whole blood sample. At a blood lead concentration of 11.2 micrograms/dL, the within- and between-run coefficients of variation were both approximately 5%. Comparison of the proposed method versus one using a recommended matrix modifier gave a regression equation of Y(proposed) = 0.99x(matrix modifier)-0.36, with a correlation coefficient of r = 0.994 (n = 54).
描述了一种用含Triton X - 100（0.1%，v/v）的0.8M（5%，v/v）硝酸溶液对全血进行脱蛋白的简单方法。所得上清液用于通过塞曼石墨炉原子吸收光谱法测定铅，采用水溶液标准品进行校准。当向脱蛋白样品上清液中加入铅水溶液时，回收率在97.7 - 105.6%之间；当向全血样品中加入全血质控物时，回收率在98.5 - 104.5%之间。在血铅浓度为11.2微克/分升时，批内和批间变异系数均约为5%。将所提出的方法与使用推荐基体改进剂的方法进行比较时，得到回归方程Y（所提出的方法）= 0.99x（基体改进剂方法） - 0.36，相关系数r = 0.994（n = 54）。
