Wickramasinghe N S, Lacey J C
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham 35294.
Mol Cell Biochem. 1994 Oct 26;139(2):117-21. doi: 10.1007/BF01081734.
Recent reports suggest that the ribosome retains considerable peptidyl transferase activity even when much of the protein of the ribosome is removed and further suggests that rRNA may be the peptidyl transferase. The work here suggests that the AMP residue at the 3' terminus of each tRNA has some catalytic activity both in the esterification reaction and in forming a pseudopeptide, AcGly, and further suggests that whatever peptidyl transferase is, it finds a cooperative substrate in the aminoacyl-AMP at the 3' terminus of tRNA.
最近的报告表明，即使核糖体的大部分蛋白质被去除，核糖体仍保留相当大的肽基转移酶活性，并且进一步表明rRNA可能就是肽基转移酶。本文的研究表明，每个tRNA 3'末端的AMP残基在酯化反应以及形成假肽AcGly方面都具有一定的催化活性，并且进一步表明，无论肽基转移酶是什么，它都能在tRNA 3'末端的氨酰-AMP中找到协同底物。
