Manukhin B N, Kichikulova T P, Moskovkin G N
Zh Evol Biokhim Fiziol. 1994 Jul-Aug;30(4):525-31.
Significant difference in values of basic parameters, Bmax and Kd which characterize binding of 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol, specific blockers of m-cholino- and beta-adrenoreceptors, has been established for brain synaptosomes from 15-day and adult rats. It was shown that phospholipase A2 (1 microgram/ml), a membrane active component from the venom of the cobra Naja naja oxiana, reduced Bmax and Kd for binding 3H-quinuclidynylbenzylate and 3H-dihydroalprenolol by synaptosomes. It is suggested that functional modifications in membrane during animal development or induced by phospholipase A2 result in shifts in the specific activity of adreno- and cholinoreceptors and, as a result, in disturbances of adrenergic and cholinergic responses.
已确定，表征间胆碱能受体和β - 肾上腺素能受体特异性阻滞剂3H - 喹核苄酯和3H - 二氢烯丙洛尔结合的基本参数Bmax和Kd值，在15日龄大鼠和成年大鼠的脑突触体之间存在显著差异。结果表明，来自眼镜蛇中亚眼镜蛇毒液的膜活性成分磷脂酶A2（1微克/毫升）降低了突触体结合3H - 喹核苄酯和3H - 二氢烯丙洛尔的Bmax和Kd。有人提出，动物发育过程中或由磷脂酶A2诱导的膜功能改变会导致肾上腺素能和胆碱能受体比活性的变化，进而导致肾上腺素能和胆碱能反应紊乱。
