Cordiali Fei P, Solmone M, Viora M, Vanacore P, Pugliese O, Giglio A, Caprilli F, Ameglio F
Laboratory of Clinical Pathology, Istituto San Gallicano, Roma, Italy.
J Biol Regul Homeost Agents. 1994 Apr-Jun;8(2):60-4.
Peripheral blood mononuclear cells from HIV-infected subjects have been demonstrated by different methods to die by apoptosis after short time in culture. In the present study the percentages of apoptotic cells have been measured by propidium iodide staining and flow cytometry in PBMC from healthy controls (15) and HIV-infected subjects with asymptomatic (10) or advanced (15) disease, with or without anti-viral treatment. The percentage of apoptosis significantly correlated with clinical stage (CDCII: 15.85% +/- 9.17, CDCIV: 22.6% +/- 5.97, P < 0.001) and the CD4/CD8 CD3 cell ratio. R = -0.57, P = 0.012), while no differences were found in relation to AZT therapy. By adding IL-2 to the cultures the percentages of apoptosis of PBMC from HIV-infected patients were significantly reduced in all experiments.
通过不同方法已证实，来自HIV感染受试者的外周血单个核细胞在培养短时间后会通过凋亡死亡。在本研究中，采用碘化丙啶染色和流式细胞术测定了健康对照者（15例）以及无症状（10例）或晚期（15例）HIV感染受试者在接受或未接受抗病毒治疗情况下外周血单个核细胞中的凋亡细胞百分比。凋亡百分比与临床分期（疾病控制中心II期：15.85%±9.17，疾病控制中心IV期：22.6%±5.97，P<0.001）以及CD4/CD8 CD3细胞比值显著相关（R=-0.57，P=0.012），而在齐多夫定治疗方面未发现差异。在所有实验中，通过向培养物中添加白细胞介素-2，HIV感染患者外周血单个核细胞的凋亡百分比显著降低。
