Björnhagen V, Bonfoco E, Brahme E M, Lindholm J, Auer G
Department of Plastic and Reconstructive Surgery, Karolinska Institute, Stockholm, Sweden.
Am J Dermatopathol. 1994 Dec;16(6):615-23. doi: 10.1097/00000372-199412000-00008.
Morphometric, DNA, and proliferating cell nuclear antigen (PCNA) measurements were taken of benign melanocytic tumors and malignant melanomas. Significant differences between lesion groups according to Krushell-Wallis analysis were found in terms of mean nuclear area, coefficient of variation (cv) of nuclear area, cv of nuclear shape nuclear contour index (NCI), mean and cv of nucleolar area, DNA 2.5 c and 5 c exceeding rates, and PCNA positivity. A logistic regression analysis with respect to banal nevi versus primary malignant melanoma showed that the cv of nuclear area and the DNA 2.5 c exceeding rate were significant independent predictors. Nuclear polymorphism, i.e., the cv of nuclear shape NCI, was larger in metastasizing primary melanomas than in thin nonmetastasizing primary melanomas. PCNA positivity was occasionally increased in keratinocytes adjacent to nevi or melanomas. Larger values for nuclear area, DNA aneuploidy, and PCNA positivity were found in thick malignant melanomas and melanoma metastases than in benign melanocytic lesions and thin malignant melanomas. Morphometry, DNA content, and PCNA positivity thus seem to reflect different stages in tumor progression of malignant melanoma.
对良性黑素细胞肿瘤和恶性黑色素瘤进行了形态测量、DNA及增殖细胞核抗原（PCNA）检测。根据Krushell-Wallis分析，发现病变组之间在平均核面积、核面积变异系数（cv）、核形态核轮廓指数（NCI）的cv、核仁面积的均值和cv、DNA 2.5c及5c超过率以及PCNA阳性率方面存在显著差异。对普通痣与原发性恶性黑色素瘤进行的逻辑回归分析表明，核面积cv和DNA 2.5c超过率是显著的独立预测指标。核多态性，即核形态NCI的cv，在发生转移的原发性黑色素瘤中比在薄的非转移性原发性黑色素瘤中更大。在痣或黑色素瘤附近的角质形成细胞中，PCNA阳性偶尔会增加。与良性黑素细胞病变和薄的恶性黑色素瘤相比，厚的恶性黑色素瘤和黑色素瘤转移灶的核面积、DNA非整倍体和PCNA阳性率的值更大。因此，形态测量、DNA含量和PCNA阳性似乎反映了恶性黑色素瘤肿瘤进展的不同阶段。
