Bergman R, Shemer A, Levy R, Friedman-Birnbaum R, Trau H, Lichtig C
Department of Dermatology, Rambam Medical Center, Haifa, Israel.
Am J Dermatopathol. 1995 Dec;17(6):547-50. doi: 10.1097/00000372-199512000-00003.
The accumulation of p53 protein was studied immunohistochemically on paraffin-embedded sections of 26 Spitz nevi (SNs), 26 primary invasive cutaneous malignant melanomas (MMs), 20 metastases of MM, and 17 ordinary compound nevi (CNs), using monoclonal antibody BP53-12. Positive reactivity was detected in some of the tumor cells in seven (35%) metastatic MMs, all exhibiting strong nuclear staining; eight (31%) primary MMs, of which seven showed strong nuclear staining; two (7%) SNs, of which only one showed strong nuclear staining; and none of the CNs. The frequencies of the positively stained lesions in general, and the strongly positively stained lesions in particular, in the MM and metastatic MM groups were each statistically significantly higher than the respective frequencies in the SN and CN groups. We believe that the immunohistochemical detection of p53 protein with the use of monoclonal antibodies such as BP53-12 on paraffin sections, especially when strong nuclear reactivity is demonstrated, may prove to be an adjunctive tool in the histopathologic differentiation of MM from SN.
使用单克隆抗体BP53 - 12，通过免疫组织化学方法研究了26例斯皮茨痣（SNs）、26例原发性浸润性皮肤恶性黑色素瘤（MMs）、20例MM转移灶以及17例普通复合痣（CNs）石蜡包埋切片中p53蛋白的积累情况。在7例（35%）MM转移灶的部分肿瘤细胞中检测到阳性反应，所有均表现为强核染色；8例（31%）原发性MM中，7例表现为强核染色；2例（7%）SNs中，仅1例表现为强核染色；而CNs均未检测到阳性反应。MM组和MM转移灶组中，一般阳性染色病变的频率，尤其是强阳性染色病变的频率，在统计学上均显著高于SN组和CN组各自的频率。我们认为，在石蜡切片上使用诸如BP53 - 12等单克隆抗体进行p53蛋白的免疫组织化学检测，特别是当出现强核反应时，可能成为MM与SN组织病理学鉴别诊断的辅助工具。
