Sirotkin A V, Nitray J, Skokanova A, Politov V P, Jurcovicova J, Marinchenko G V
Research Institute of Animal Production, Nitra, Slovakia.
Ann Endocrinol (Paris). 1994;55(6):279-82.
The time-dependent accumulation of prolactin (PRL) immunoreactivity in serum-free and in serum-supplemented cultures of bovine granulosa cells was observed. Blockade of endogenous progesterone by specific antiserum had no significant influence on PRL immunoreactivity, but progesterone addition (10-10,000 ng/ml) lead to a dose-dependent decrease. Antisera against testosterone and against estradiol-17 beta were inhibitors, and additions of these hormones (100-10,000 ng/ml) stimulated the release of PRL immunoreactivity. The results obtained suggest the secretion of a PRL-like peptide by bovine ovarian cells. Growth hormone, testosterone and estradiol may be stimulators, and progesterone a possible inhibitor of this process.
观察了无血清和添加血清培养的牛颗粒细胞中催乳素（PRL）免疫反应性随时间的积累情况。用特异性抗血清阻断内源性孕酮对PRL免疫反应性无显著影响，但添加孕酮（10 - 10000 ng/ml）会导致剂量依赖性降低。抗睾酮和抗雌二醇-17β的抗血清是抑制剂，添加这些激素（100 - 10000 ng/ml）会刺激PRL免疫反应性的释放。所得结果表明牛卵巢细胞分泌一种PRL样肽。生长激素、睾酮和雌二醇可能是该过程的刺激物，而孕酮可能是抑制剂。
