Ishibashi T, Inomata H, Sakamoto T, Ryan S J
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Arch Ophthalmol. 1995 Feb;113(2):227-31. doi: 10.1001/archopht.1995.01100020111041.
To examine the role of pericytes in the progression of subretinal neovascularization in a primate model.
Subretinal neovascularization was induced by intense laser photocoagulation in four monkey eyes. Single eyes were enucleated at 3, 7, 14, and 21 days after photocoagulation and were studied with light and electron microscopy.
Three days after photocoagulation, newly formed vessels were observed in the choroid and subretinal space. Although most of these vessels had an immature appearance and consisted only of endothelial cells that formed narrow lumens, mitotic figures of pericytes were occasionally found near the endothelial cells. Seven days after photocoagulation, all of the newly formed vessels possessed pericytes. Fourteen days after photocoagulation, many vessels appeared to be mature. Many sites of pericyte-endothelial cell contact were observed. These contacts were composed of cytoplasmic interdigitation and membrane apposition. By 21 days after photocoagulation, the mature vessels had increased in number, and the endothelial cells had many fenestrations with diaphragms. The pericyte coverage of the endothelial cells was less at this stage than at 14 days after photocoagulation, and sites of pericyte-endothelial cell contacts were observed only rarely.
Pericytes are involved in maturation of the endothelial cells that form subretinal new vessels.
在灵长类动物模型中研究周细胞在视网膜下新生血管形成过程中的作用。
用强激光光凝法诱导4只猴眼形成视网膜下新生血管。光凝后3、7、14和21天摘除单眼，进行光镜和电镜研究。
光凝后3天，在脉络膜和视网膜下间隙观察到新形成的血管。虽然这些血管大多外观不成熟，仅由形成狭窄管腔的内皮细胞组成，但在内皮细胞附近偶尔可见周细胞的有丝分裂象。光凝后7天，所有新形成的血管都有周细胞。光凝后14天，许多血管似乎已成熟。观察到许多周细胞与内皮细胞接触的部位。这些接触由细胞质交错和膜贴附组成。到光凝后21天，成熟血管数量增加，内皮细胞有许多带隔膜的窗孔。此时内皮细胞的周细胞覆盖比光凝后14天时少，仅偶尔观察到周细胞与内皮细胞接触的部位。
周细胞参与形成视网膜下新血管的内皮细胞的成熟过程。
