绵羊胰腺微粒体作为研究蛋白质转运的犬源替代物。
Sheep pancreatic microsomes as an alternative to the dog source for studying protein translocation.
作者信息
Kaderbhai M A, Harding V J, Karim A, Austen B M, Kaderbhai N N
机构信息
Institute of Biological Sciences, University of Wales, Penglais, Aberystwyth, U.K.
出版信息
Biochem J. 1995 Feb 15;306 ( Pt 1)(Pt 1):57-61. doi: 10.1042/bj3060057.
Abstract
A procedure is described for the preparation of rough membrane vesicles of endoplasmic-reticular origin from the pancreas of sheep. These isolated membranes translocate, process and glycosylate in vitro-translated heterologous proteins in a manner comparable with that exhibited by dog pancreatic microsomes.
摘要
本文描述了一种从绵羊胰腺制备内质网来源的粗膜囊泡的方法。这些分离的膜能够以与犬胰腺微粒体类似的方式，在体外对翻译后的异源蛋白质进行转运、加工和糖基化。
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