Li H, Akamizu T, Okuda J, Sugawa H, Matsuda F, Tsubata T, Mori T
Department of Laboratory Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1995 Feb 27;207(3):985-93. doi: 10.1006/bbrc.1995.1282.
In autoimmune diseases, IgG class autoantibodies are generally considered to be more pathognomonic than IgM class ones. Although Epstein-Barr virus (EBV)-transformation of lymphocytes is a useful method to obtain human monoclonal autoantibodies, it tends to result predominantly in IgM-producing cells. We depleted IgM+ cells before EBV-transformation with a Magnetic Cell Separator (MACS) in order to increase the chance of acquisition of cells producing IgG class anti-thyrotropin (TSH) receptor antibodies (TRAb). As a result, we obtained four independent B cell clones producing IgG class monoclonal thyroid-stimulating antibodies (TSAb) from three patients with Graves' disease. None of these clones showed any TSH binding inhibitor immunoglobulin (TBII) activity, suggesting independence of TSAb-producing lymphocytes from those producing TBII.
在自身免疫性疾病中，IgG类自身抗体通常被认为比IgM类自身抗体更具疾病特征性。尽管淋巴细胞的爱泼斯坦-巴尔病毒（EBV）转化是获得人单克隆自身抗体的一种有用方法，但它往往主要产生分泌IgM的细胞。为了增加获得产生IgG类抗促甲状腺激素（TSH）受体抗体（TRAb）细胞的机会，我们在EBV转化前用磁性细胞分离器（MACS）去除了IgM+细胞。结果，我们从三名格雷夫斯病患者中获得了四个独立的产生IgG类单克隆甲状腺刺激抗体（TSAb）的B细胞克隆。这些克隆均未显示出任何促甲状腺激素结合抑制免疫球蛋白（TBII）活性，这表明产生TSAb的淋巴细胞与产生TBII的淋巴细胞相互独立。
