Burt D W, Dey B R, Paton I R, Morrice D R, Law A S
Division of Molecular Biology, Roslin Institute Edinburgh, Midlothian, UK.
DNA Cell Biol. 1995 Feb;14(2):111-23. doi: 10.1089/dna.1995.14.111.
In this paper, we report the isolation, characterization, and mapping of the chicken transforming growth factor-beta 3 (TGF-beta 3) gene. The gene contains seven exons and six introns spanning 16-kb of the chicken genome. A comparison of the 5'-flanking regions of human and chicken TGF-beta 3 genes reveals two regions of sequence conservation. The first contains ATF/CRE and TBP/TATA sequence motifs within an 87-bp region. The second is a 162-bp region with no known sequence motifs. Identification of transcription start sites using chicken RNA isolated from various embryonic and adult tissues reveals two sites of initiation, P1 and P2, which map to these two conserved regions. Comparison of 3'-flanking regions of chicken and mammalian TGF-beta 3 genes also revealed conserved sequences. The most significant homologies were found in the 3'-most end of the transcribed region. DNA sequence analysis of chicken TGF-beta 3 cDNAs isolated by 3'-RACE revealed multiple polyadenylation sites unusually distant from a poly(A) signal motif. A Msc I restriction fragment length polymorphism (RFLP) marker was used to map the TGFB3 locus to linkage group E7 on the East Lansing reference backcross. Linkage to the TH locus showed that the TGFB3 locus was physically located on chicken chromosome 5.
在本文中，我们报道了鸡转化生长因子β3（TGF-β3）基因的分离、特征分析及定位。该基因包含7个外显子和6个内含子，跨越鸡基因组的16 kb。对人和鸡TGF-β3基因5'侧翼区域的比较揭示了两个序列保守区域。第一个区域在一个87 bp的区域内包含ATF/CRE和TBP/TATA序列基序。第二个区域是一个162 bp的区域，没有已知的序列基序。使用从各种胚胎和成年组织中分离的鸡RNA鉴定转录起始位点，发现了两个起始位点P1和P2，它们定位于这两个保守区域。对鸡和哺乳动物TGF-β3基因3'侧翼区域的比较也揭示了保守序列。在转录区域的最3'端发现了最显著的同源性。通过3'-RACE分离的鸡TGF-β3 cDNA的DNA序列分析揭示了多个聚腺苷酸化位点，这些位点距离聚（A）信号基序异常遥远。一个Msc I限制性片段长度多态性（RFLP）标记被用于将TGFB3基因座定位到东兰辛参考回交群体的E7连锁群上。与TH基因座的连锁表明TGFB3基因座实际位于鸡的5号染色体上。
