Froebel K S, Aldhous M C, Mok J Y, Hayley J, Arnott M, Peutherer J F
Department of Medicine, University of Edinburgh, United Kingdom.
AIDS Res Hum Retroviruses. 1994;10 Suppl 2:S83-8.
Of the Edinburgh cohort of approximately 130 children born to HIV-infected women, 9 are infected and alive. This article describes results from the first 18 months of a natural history study of seven of these, and two adopted children, studying the CD8 T cell-mediated cytotoxicity against HIV proteins (Gag, Tat, Pol, and Env), over time, and relating it to clinical progression and viral activity. Autologous EBV cell lines infected with vaccinia-HIV constructs were used as target cells, and bulk-cultured peripheral blood mononuclear cells as effector cells. The children ranged in age from 0 to 93 months, with six of the nine showing CTL activity to one or more HIV proteins. The specificity of the response was directed against Tat in the younger children, switching to Pol, then Gag or Env. Preliminary analysis of virological data showed no association between CTL and virus activity. The children with CTLs tended to be well clinically, but the cohort needs to be studied longer before conclusions can be made about CTL activity and HIV disease progression. Cytotoxic T lymphocyte activity has also been observed in two children diagnosed as HIV uninfected. These results show the importance of looking at CTL specificity, and may have implications in vaccine design.
在爱丁堡，约130名感染艾滋病毒的妇女所生儿童中，有9名感染了艾滋病毒且仍然存活。本文描述了其中7名儿童以及2名领养儿童自然史研究头18个月的结果，该研究旨在研究随着时间推移，CD8 T细胞对艾滋病毒蛋白（Gag、Tat、Pol和Env）的细胞毒性作用，并将其与临床进展和病毒活性相关联。用感染痘苗 - 艾滋病毒构建体的自体EBV细胞系作为靶细胞，用大量培养的外周血单核细胞作为效应细胞。这些儿童年龄从0到93个月不等，9名儿童中有6名对一种或多种艾滋病毒蛋白表现出CTL活性。年幼儿童的反应特异性针对Tat，随后转向Pol，然后是Gag或Env。病毒学数据的初步分析显示CTL与病毒活性之间没有关联。有CTL的儿童临床状况往往良好，但在对CTL活性和艾滋病毒疾病进展得出结论之前，还需要对该队列进行更长时间的研究。在两名被诊断为未感染艾滋病毒的儿童中也观察到了细胞毒性T淋巴细胞活性。这些结果表明了研究CTL特异性的重要性，并且可能对疫苗设计有影响。
