Mattar S, Centella A, Daza C, García J
Departamento de Microbiología, Universidad Javeriana, Facultad de Ciencias, Bogotá D.C., Columbia.
Enferm Infecc Microbiol Clin. 1994 Dec;12(10):484-9.
Acute gastroenteritis (AGE) is one of the most common diseases in children, particularly in those under the age of 5 years in whom a severe picture of hydroelectrolyte imbalance may be triggered accompanied, in most cases, by leukocyte response. The aim of this study was to evaluate three new culture media for the isolation of enteropathogens in infantile diarrhea.
From April to September, 1993, 170 samples of diarrhea stools from children up to 7 years of age attending pediatric hospitals in Bogota were studied. Three new culture media were used in the isolation of the enteropathogenic bacteria: BGLN, MILA agar, Rambach agar and a conventional agar medium S.S. Biochemical tests were performed to identify the isolated bacteria.
A total of 98.5% of enteropathogenic E. coli was isolated in Rambach agar, which also presented excellent recovery rates of Salmonella sp. (100%) versus S.S> (64.3%) and BGLN (77.2%), respectively. The best selectivity for Shigella sp. was observed with BGLN with a 100% recovery rate. Out of the 170 samples 105 showed a leukocyte count of 70-75% and positive isolation for enteropathogenic bacteria. Six samples with the same leukocyte count did not present enteropathogenic bacteria, with the 59 remaining samples with a 20-25% PMN count being negative for enteropathogenic bacteria.
The results suggest that the new culture media used in this study may have better recovery rates for enteropathogenic bacteria in acute gastroenteritis. Likewise, a correlation was observed between leukocyte count and isolation enteropathogenic bacteria.
急性肠胃炎（AGE）是儿童最常见的疾病之一，尤其是5岁以下的儿童，这类儿童可能会引发严重的水电解质失衡，多数情况下还伴有白细胞反应。本研究的目的是评估三种用于分离婴幼儿腹泻中肠道病原体的新培养基。
1993年4月至9月，对波哥大儿童医院7岁以下儿童的170份腹泻粪便样本进行了研究。使用三种新培养基分离肠道致病菌：BGLN、米拉琼脂、兰巴赫琼脂和传统琼脂培养基SS。进行生化试验以鉴定分离出的细菌。
兰巴赫琼脂中分离出的肠道致病性大肠杆菌占总数的98.5%，该培养基对沙门氏菌属的回收率也很高（100%），而SS培养基和BGLN培养基的回收率分别为64.3%和77.2%。观察到BGLN对志贺氏菌属的选择性最佳，回收率为100%。在170份样本中, 105份样本的白细胞计数为70 - 75%，肠道致病菌分离呈阳性。6份白细胞计数相同的样本未检出肠道致病菌，其余59份中性粒细胞计数为20 - 25%的样本肠道致病菌检测呈阴性。
结果表明，本研究中使用的新培养基对急性肠胃炎中肠道致病菌的回收率可能更高。同样，观察到白细胞计数与肠道致病菌分离之间存在相关性。
