使用兰巴赫琼脂和NBGL琼脂对4037份人类粪便样本及584份提交用于沙门氏菌分离的兽医样本进行研究。

Study of Rambach and NBGL agar on 4037 stools of human origin and 584 veterinary samples submitted for isolation of Salmonellae.

作者信息

Poisson D M, Nugier J P, Rousseau P

机构信息

Laboratoire de Microbiologie, CHR Orléans, France.

出版信息

Pathol Biol (Paris). 1993 Jun;41(6):543-6.

PMID:8247634

Abstract

Rambach agar and novobiocin-brilliant green-glycerol-lactose (NBGL) agar are two recently described media designed for Salmonella isolation. Rambach and NBGL agars were compared to usual media in human and veterinary routines, by evaluation of sensitivities and predictive values of suspect colonies. In the human routine, 4037 stools, sensitivities were 79% for Hektoen agar, 51% for Rambach agar and 96% for NBGL agar at the direct plating for a total of 94 strains; and 89%, 87% and 94% respectively at the plating of Mueller-Kauffmann enrichment broth for a total of 143 strains. Predictive values of suspect colonies were respectively 58%, 93%, 91% at the direct plating; and 25%, 74%, 80% at the plating of Mueller-Kauffmann enrichment broth. In the veterinary routine, 584 samples, sensitivity was 65% on the association of Salmonella-Shigella and Hektoen agars, 70% on Rambach agar and 85% on NBGL agar, for a total of 40 strains. Predictive values of suspect colonies were 7.4%, 82.3% and 83% respectively. These results showed that Rambach and NBGL agars have distinct properties, and are both highly predictive. The highest sensitivities were achieved by the NBGL agar.

摘要

兰巴赫琼脂和新生霉素-亮绿-甘油-乳糖（NBGL）琼脂是最近描述的两种用于分离沙门氏菌的培养基。通过评估可疑菌落的敏感性和预测值，将兰巴赫琼脂和NBGL琼脂与人类和兽医常规使用的常用培养基进行了比较。在人类常规检测中，对4037份粪便样本进行检测，直接接种时，对于总共94株菌株，赫克托恩琼脂的敏感性为79%，兰巴赫琼脂为51%，NBGL琼脂为96%；在接种穆勒-考夫曼增菌肉汤后，对于总共143株菌株，敏感性分别为89%、87%和94%。可疑菌落的预测值在直接接种时分别为58%、93%、91%；在接种穆勒-考夫曼增菌肉汤后分别为25%、74%、80%。在兽医常规检测中，对584份样本进行检测，对于总共40株菌株，沙门氏菌-志贺氏菌琼脂和赫克托恩琼脂联合使用时的敏感性为65%，兰巴赫琼脂为70%，NBGL琼脂为85%。可疑菌落的预测值分别为7.4%、82.3%和83%。这些结果表明，兰巴赫琼脂和NBGL琼脂具有不同的特性，且预测性都很高。NBGL琼脂的敏感性最高。