编码猫CD8α基因的cDNA的稳定表达。

Stable expression of the cDNA encoding the feline CD8 alpha gene.

作者信息

Pecoraro M R, Kawaguchi Y, Okita M, Inoshima Y, Tohya Y, Kai C, Mikami T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1994 Oct;56(5):1001-3. doi: 10.1292/jvms.56.1001.

DOI:10.1292/jvms.56.1001

PMID:7865571

Abstract

The stable expression of the alpha chain of the feline cytotoxic T cell differentiation antigen (fCD8 alpha) on Crandell feline kidney cells (CRFK) was carried out by using an expression vector which contains the Rous sarcoma virus long terminal repeat and a neo resistant gene. After three rounds of cloning under G418 selection for over two months, the expression of the feline polypeptide was detected by human monoclonal antibody OKT8.

摘要

利用一种含有劳氏肉瘤病毒长末端重复序列和新霉素抗性基因的表达载体，在克兰德尔猫肾细胞（CRFK）上实现了猫细胞毒性T细胞分化抗原（fCD8α）α链的稳定表达。在G418选择下进行三轮克隆，历时两个多月后，用人单克隆抗体OKT8检测到了猫多肽的表达。