抗猫 CD8 单克隆抗体的特性分析

Characterization of anti-feline CD8 monoclonal antibodies.

作者信息

Shimojima M, Pecoraro M R, Maeda K, Tohya Y, Miyazawa T, Mikami T

机构信息

Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

出版信息

Vet Immunol Immunopathol. 1998 Feb 16;61(1):17-23. doi: 10.1016/s0165-2427(97)00091-3.

DOI:10.1016/s0165-2427(97)00091-3

PMID:9613469

Abstract

We generated three monoclonal antibodies (mAbs) (2D7, 10C7 and 12A3) reactive to the alpha-chain of feline CD8 (fCD8) molecule. Further we showed that reference anti-fCD8 mAbs, FT2, 3.357 and vpg9 recognize the beta-chain, alpha-chain and alphabeta-complex epitope, respectively. Flow cytometric analysis using these mAbs suggested that fCD8alpha(+)beta(-) cells were present in lymphocytes of spleen, but not significantly in those of thymus, lymph nodes and peripheral blood of normal kittens.

摘要

我们制备了三种对猫 CD8（fCD8）分子的α链具有反应性的单克隆抗体（mAb）（2D7、10C7 和 12A3）。此外，我们还表明，参考抗 fCD8 mAb，FT2、3.357 和 vpg9 分别识别β链、α链和αβ复合表位。使用这些 mAb 进行的流式细胞术分析表明，fCD8α(+)β(-)细胞存在于正常小猫脾脏的淋巴细胞中，但在胸腺、淋巴结和外周血的淋巴细胞中不显著。