Langlois M R, Delanghe J R, Kaufman J M, De Buyzere M L, Van Hoecke M J, Leroux-Roels G G
Central Laboratory, University Hospital, Gent, Belgium.
Eur J Clin Chem Clin Biochem. 1994 Sep;32(9):675-80. doi: 10.1515/cclm.1994.32.9.675.
Bone alkaline phosphatase is a marker of osteoblast activity. In order to study the posttranscriptional modification (glycosylation) of bone alkaline phosphatase in bone disease, we investigated the relationship between mass and catalytic activity of bone alkaline phosphatase in patients with osteoporosis and hyperthyroidism. Serum bone alkaline phosphatase activity was measured after lectin precipitation using the Iso-ALP test kit. Mass concentration of bone alkaline phosphatase was determined with an immunoradiometric assay (Tandem-R Ostase). In general, serum bone alkaline phosphatase mass and activity concentration correlated well. The activity : mass ratio of bone alkaline phosphatase was low in hyperthyroidism. Activation energy of the reaction catalysed by bone alkaline phosphatase was high in osteoporosis and in hyperthyroidism. Experiments with neuraminidase digestion further demonstrated that the thermodynamic heterogeneity of bone alkaline phosphatase can be explained by a different glycosylation of the enzyme.
骨碱性磷酸酶是成骨细胞活性的标志物。为了研究骨病中骨碱性磷酸酶的转录后修饰（糖基化），我们调查了骨质疏松症和甲状腺功能亢进症患者骨碱性磷酸酶的质量与催化活性之间的关系。使用Iso-ALP检测试剂盒通过凝集素沉淀法测定血清骨碱性磷酸酶活性。采用免疫放射分析（串联-R骨碱性磷酸酶检测法）测定骨碱性磷酸酶的质量浓度。一般来说，血清骨碱性磷酸酶质量与活性浓度具有良好的相关性。甲状腺功能亢进症患者骨碱性磷酸酶的活性：质量比很低。骨质疏松症和甲状腺功能亢进症患者中，由骨碱性磷酸酶催化的反应的活化能较高。神经氨酸酶消化实验进一步证明，骨碱性磷酸酶的热力学异质性可以通过该酶不同的糖基化来解释。
