Virgili M, Facchinetti F, Ciani E, Contestabile A
Department of Biology, University of Bologna, Italy.
Neuroreport. 1994 Oct 27;5(16):2141-4. doi: 10.1097/00001756-199410270-00038.
Neonatal rats were daily treated with increasing doses of the competitive NMDA antagonist CGP 39551 from postnatal day 1 to 22. At 75-80 days of age the rats were given an excitotoxic dose of kainic acid s.c. Histological examination suggested that the olfactory cortex of the neonatally CGP 39551-treated rats was less damaged than that of controls. This was quantitatively confirmed by assaying the decrease of appropriate neurochemical markers (D-[3H]aspartate uptake and glutamate decarboxylase) as an index of the extent of neuronal degeneration. By contrast, the decrease of the same markers was not different in the hippocampus. These results suggest a selective effect on some brain circuits of adult rats consequent to the neonatal blockade of NMDA receptors and, therefore, add new evidence to a developmental role of this receptor.
从出生后第1天至第22天，每天给新生大鼠注射递增剂量的竞争性N-甲基-D-天冬氨酸（NMDA）拮抗剂CGP 39551。在75 - 80日龄时，给大鼠皮下注射兴奋性毒性剂量的红藻氨酸。组织学检查表明，新生期接受CGP 39551治疗的大鼠嗅皮质的损伤程度低于对照组。通过检测适当的神经化学标志物（D-[3H]天冬氨酸摄取和谷氨酸脱羧酶）的减少作为神经元变性程度的指标，这一点得到了定量证实。相比之下，相同标志物在海马体中的减少没有差异。这些结果表明，新生期NMDA受体阻断对成年大鼠的某些脑回路有选择性作用，因此，为该受体的发育作用增添了新的证据。
