Kershaw T R, Rashid-Doubell F, Sinden J D
Neurodegenerative Diseases Research Group, Institute of Psychiatry, De Crespigny Park, London, UK.
Neuroreport. 1994 Oct 27;5(16):2197-200. doi: 10.1097/00001756-199410270-00052.
From dissected fragments of embryonic H-2Kb-tsA58 transgenic mouse hippocampal neuroepithelium, we have derived a population of rapidly proliferating, nestin-positive conditionally immortal hippocampal neuroepithelial cells. Treatment with dibutyryl cAMP in non-permissive culture conditions resulted in cessation of cell division and differentiation of the precursor cells into neuronal or glial phenotypes.
从胚胎期H-2Kb-tsA58转基因小鼠海马神经上皮的解剖碎片中，我们获得了一群快速增殖、巢蛋白阳性的条件性永生化海马神经上皮细胞。在非允许培养条件下用二丁酰环磷腺苷处理导致细胞分裂停止，前体细胞分化为神经元或神经胶质表型。
