Dennis J E, Caplan A I
Biology Department, Case Western Reserve University, Cleveland, OH 44106, USA.
Connect Tissue Res. 1996;35(1-4):93-9. doi: 10.3109/03008209609029179.
All nucleated cells from the adult transgenic mouse H-2Kb-tsA58 harbor the temperature-sensitive mutant gene for SV40 large T-antigen. Bone marrow cells from this transgenic mouse were isolated, expanded and cloned in vitro under conditions permissive to the expression of stable T-antigen. Clones of marrow-derived mesenchymal progenitor cells were tested in vitro and in vivo for their capacity to differentiate into mature mesenchymal phenotypes of bone, cartilage, muscle, adipose, and hematopoietic support cells (termed "stromacytes"). Mono-, bi-, and tri-potential clones were identified that were able to differentiate into bone, adipose and stromacyte phenotypes. A mixed population of cells showed some chondrocytic potential in vivo, however, no evidence of cartilage matrix production was detected in vitro for any of the immortomouse clones. These results support the hypothesis that marrow contains multiple progenitor cells that are part of a mesenchymal lineage.
成年转基因小鼠H-2Kb-tsA58的所有有核细胞都携带SV40大T抗原的温度敏感突变基因。从这种转基因小鼠中分离出骨髓细胞，在允许稳定T抗原表达的条件下进行体外扩增和克隆。对骨髓来源的间充质祖细胞克隆进行体外和体内测试，以检测它们分化为骨、软骨、肌肉、脂肪和造血支持细胞（称为“基质细胞”）等成熟间充质表型的能力。鉴定出了能够分化为骨、脂肪和基质细胞表型的单能、双能和三能克隆。一群混合细胞在体内显示出一些软骨细胞潜能，然而，在体外未检测到任何永生小鼠克隆产生软骨基质的证据。这些结果支持了骨髓中含有多个属于间充质谱系的祖细胞这一假说。
