Chao Y C, Tang H S, Hsu C T
Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C.
J Formos Med Assoc. 1994 Jul;93(7):562-8.
The partial genome sequence of the hepatitis C virus (HCV) was determined in the serum of a Taiwanese patient with chronic community-acquired type C hepatitis. The cDNA fragments synthesized with the HCV RNA as a template were amplified by polymerase chain reaction using specific oligonucleotide primers. The amplified fragments represented the regions coding for the putative core, matrix and envelope proteins as well as the N-terminal amino acid sequence of the nonstructural protein NS1, the partial nonstructural NS3 and NS4 proteins and the region of the partial 5'-end noncoding sequence. The cDNA fragments were cloned and sequenced. Sequence analysis of these clones showed that they share 83.7%, 93.2% and 93.6% similarity at the nucleotide level, and 86.6%, 94.1% and 92.9% homology at the amino acid level, with the previously published American, Japanese and Taiwanese isolates, respectively. Accordingly, the RNA genome we obtained is HCV type II, probably, the predominant subtype in Taiwan.
在一名患有慢性社区获得性丙型肝炎的台湾患者血清中测定了丙型肝炎病毒（HCV）的部分基因组序列。以HCV RNA为模板合成的cDNA片段，使用特异性寡核苷酸引物通过聚合酶链反应进行扩增。扩增片段代表了编码假定的核心蛋白、基质蛋白和包膜蛋白的区域，以及非结构蛋白NS1的N端氨基酸序列、部分非结构NS3和NS4蛋白以及部分5'-端非编码序列区域。将cDNA片段进行克隆和测序。这些克隆的序列分析表明，它们在核苷酸水平上分别与先前发表的美国、日本和台湾分离株具有83.7%、93.2%和93.6%的相似性，在氨基酸水平上分别具有86.6%、94.1%和92.9%的同源性。因此，我们获得的RNA基因组可能是HCV II型，可能是台湾的主要亚型。
