Tokuda H, Sato E, Kurokawa T, Kusunoki N, Ishibashi S
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Biochem Mol Biol Int. 1994 Oct;34(4):653-9.
It is well known that forskolin suppresses glucose transport in cell membranes. In the present study, however, we indicated a different effect of forskolin on the uptake of radioactive 2-deoxy-D-glucose (2-DG) by cultured fibroblasts between the values measured in the presence of unlabeled 2-DG and those measured in the presence of unlabeled glucose. In the former case, the uptake was inhibited by forskolin. On the contrary, [3H] 2-DG uptake in the presence of unlabeled glucose was increased by forskolin. These results suggest that the metabolic difference between the two sugars is involved in the above-mentioned difference in the effect of forskolin. This paper presents that forskolin has stimulatory effect on glycolysis in addition to an inhibitory effect on glucose transporter. The findings seem to be of another interest to indicate the relationship between membrane transport and subsequent intracellular metabolism of glucose.
众所周知，福斯高林可抑制细胞膜中的葡萄糖转运。然而，在本研究中，我们发现福斯高林对培养的成纤维细胞摄取放射性2-脱氧-D-葡萄糖（2-DG）的影响，在未标记的2-DG存在下测得的值与未标记的葡萄糖存在下测得的值之间存在差异。在前一种情况下，福斯高林抑制摄取。相反，在未标记的葡萄糖存在下，[3H]2-DG的摄取会因福斯高林而增加。这些结果表明，两种糖之间的代谢差异与上述福斯高林作用的差异有关。本文提出，福斯高林除了对葡萄糖转运蛋白有抑制作用外，对糖酵解还有刺激作用。这些发现似乎还有另一个有趣之处，即表明膜转运与随后葡萄糖的细胞内代谢之间的关系。
