Lee T P, Park B H
Res Commun Chem Pathol Pharmacol. 1981 Feb;31(2):275-83.
Rat thymocyte glucose transport activity was measured by the uptake of 3H-2-deoxyglucose. In the presence of 5mM glucose, DNP stimulated the uptake of 2-deoxyglucose. In the absence of glucose, DNP inhibited the uptake of 2-deoxyglucose. Both stimulatory and inhibitor effects of DNP were DNP concentration dependent. The incubation of thymocytes with nutrients which are metabolized through mitochondrial oxidation, such as beta-hydroxybutyrate, pyruvate, alanine and glutamine, did not support the stimulatory effects of DNP on 2-deoxyglucose uptake. The incubation of thymocytes with a nutrient which can support glycolysis, such as adenosine, supported the stimulatory effect of DNP on 2-deoxyglucose uptake. Iodoacetate, an inhibitor of glycolysis, inhibited 2-deoxyglucose uptake even in the presence of glucose. The present data suggested that the stimulatory effect of DNP on thymocyte 2-deoxyglucose uptake required intact glycolysis activity.
通过3H-2-脱氧葡萄糖的摄取来测量大鼠胸腺细胞的葡萄糖转运活性。在5mM葡萄糖存在的情况下，二硝基苯酚（DNP）刺激2-脱氧葡萄糖的摄取。在没有葡萄糖的情况下，DNP抑制2-脱氧葡萄糖的摄取。DNP的刺激和抑制作用均取决于DNP的浓度。用可通过线粒体氧化代谢的营养物质（如β-羟基丁酸酯、丙酮酸、丙氨酸和谷氨酰胺）孵育胸腺细胞，不支持DNP对2-脱氧葡萄糖摄取的刺激作用。用可支持糖酵解的营养物质（如腺苷）孵育胸腺细胞，支持DNP对2-脱氧葡萄糖摄取的刺激作用。碘乙酸是糖酵解的抑制剂，即使在有葡萄糖存在的情况下也能抑制2-脱氧葡萄糖的摄取。目前的数据表明，DNP对胸腺细胞2-脱氧葡萄糖摄取的刺激作用需要完整的糖酵解活性。
