Pimenta P F, Touray M, Miller L
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
J Eukaryot Microbiol. 1994 Nov-Dec;41(6):608-24. doi: 10.1111/j.1550-7408.1994.tb01523.x.
The life cycle of malaria parasites in the mosquito vector is completed when the sporozoites infect the salivary gland and are ready to be injected into the vertebrate host. This paper describes the fine structure of the invasive process of mosquito salivary glands by malaria parasites. Plasmodium gallinaceum sporozoites start the invasion process by attaching to and crossing the basal lamina and then penetrating the host plasma membrane of the salivary cells. The penetration process appears to involve the formation of membrane junctions. Once inside the host cells, the sporozoites are seen within vacuoles attached by their anterior end to the vacuolar membrane. Mitochondria surround, and are closely associated with, the invading sporozoites. After the disruption of the membrane vacuole, the parasites traverse the cytoplasm, attach to, and invade the secretory cavity through the apical plasma membrane of the cells. Inside the secretory cavity, sporozoites are seen again inside vacuoles. Upon escaping from these vacuoles, sporozoites are positioned in parallel arrays forming large bundles attached by multilammelar membrane junctions. Several sporozoites are seen around and inside the secretory duct. Except for the penetration of the chitinous salivary duct, our observations have morphologically characterized the entire process of sporozoite passage through the salivary gland.
当子孢子感染唾液腺并准备好注入脊椎动物宿主时，疟原虫在蚊媒中的生命周期就完成了。本文描述了疟原虫侵入蚊唾液腺过程的精细结构。鸡疟原虫的子孢子通过附着并穿过基膜，然后穿透唾液细胞的宿主质膜开始侵入过程。穿透过程似乎涉及膜连接的形成。一旦进入宿主细胞，子孢子就出现在液泡内，其前端附着在液泡膜上。线粒体围绕着侵入的子孢子并与其紧密相关。膜泡破裂后，寄生虫穿过细胞质，附着并通过细胞的顶端质膜侵入分泌腔。在分泌腔内，又可见到子孢子在液泡内。从这些液泡中逸出后，子孢子以平行排列的方式定位，形成由多层膜连接附着的大束。在分泌管周围和内部可见到几个子孢子。除了几丁质唾液管的穿透外，我们的观察从形态学上描述了子孢子通过唾液腺的整个过程。
