新西兰肥胖小鼠棕色脂肪组织中GLUT4蛋白水平早期下降。

Early decrease in GLUT4 protein levels in brown adipose tissue of New Zealand obese mice.

作者信息

Ferreras L, Kelada A S, McCoy M, Proietto J

机构信息

Department of Medicine (Royal Melbourne Hospital), University of Melbourne, Parkville, Australia.

出版信息

Int J Obes Relat Metab Disord. 1994 Nov;18(11):760-5.

PMID:7866477

Abstract

The aim of this study was to determine if the previously described insulin resistance in the New Zealand Obese (NZO) mouse is associated with a decrease in GLUT4 protein and if such changes occur early in the evolution of the syndrome. GLUT4 levels were measured in whole membranes isolated from a variety of tissues in 4 and 20-week-old NZO and control NZC mice by Western blotting using a specific antibody to the C terminal end of the protein. At 20 weeks of age, GLUT4 levels were lower in the NZO mice in brown and white adipose tissue, heart, diaphragm, red and white quadriceps, and red and white gastrocnemius, but not in soleus muscle. At 4 weeks of age, GLUT4 levels were 52% lower in BAT (3309 +/- 1006 vs 6951 +/- 1870 cpm P = 0.039) but were not lower in WAT, heart or red quadriceps. It is concluded that adult NZO mice have a decrease in GLUT4 levels in most insulin-sensitive tissues and that in BAT, this occurs at an early age.

摘要

本研究的目的是确定先前描述的新西兰肥胖（NZO）小鼠中的胰岛素抵抗是否与葡萄糖转运蛋白4（GLUT4）水平降低有关，以及这种变化是否在该综合征演变的早期就会出现。使用针对该蛋白C末端的特异性抗体，通过蛋白质免疫印迹法，测定4周龄和20周龄的NZO小鼠及对照NZC小鼠各种组织分离出的全细胞膜中的GLUT4水平。在20周龄时，NZO小鼠的棕色和白色脂肪组织、心脏、膈肌、红、白股四头肌以及红、白腓肠肌中的GLUT4水平较低，但比目鱼肌中没有降低。在4周龄时，棕色脂肪组织中的GLUT4水平降低了52%（3309±1006对6951±1870 cpm，P = 0.039），但白色脂肪组织、心脏或红股四头肌中的GLUT4水平没有降低。得出的结论是，成年NZO小鼠大多数胰岛素敏感组织中的GLUT4水平降低，并且在棕色脂肪组织中，这种情况在早期就会发生。