气相色谱-质谱联用测定人血清中的硝苯地平：方法验证及其在生物利用度研究中的应用
Determination of nifedipine in human serum by gas chromatography-mass spectrometry: validation of the method and its use in bioavailability studies.
作者信息
Martens J, Banditt P, Meyer F P
机构信息
Department of Clinical Pharmacology, University Hospital, Otto-von-Guericke University Magdeburg, Germany.
出版信息
J Chromatogr B Biomed Appl. 1994 Oct 14;660(2):297-302. doi: 10.1016/0378-4347(94)00289-4.
A procedure for the determination of nifedipine in human serum is described. The light-sensitive substance is isolated from serum by liquid-liquid extraction and analyzed using capillary gas chromatography with a mass-selective detector. The validation of the method shows that the extraction recovery is ca. 85%, the limit of detection is 2 ng/ml and the standard deviations of the intra-day precision test range from 5.8 to 7.4% with respect to the concentration. The procedure is highly selective and sensitive. It is especially suited for bioavailability studies because of its stability and high sampling rate.
描述了一种测定人血清中硝苯地平的方法。通过液-液萃取从血清中分离出光敏物质，并使用带质量选择检测器的毛细管气相色谱法进行分析。该方法的验证表明，萃取回收率约为85%，检测限为2 ng/ml，日内精密度测试的相对浓度标准偏差范围为5.8%至7.4%。该方法具有高度的选择性和灵敏性。由于其稳定性和高采样率，它特别适用于生物利用度研究。
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