Wu J, Zhang Z X, Wang Y
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009.
Yao Xue Xue Bao. 1997;32(2):147-50.
A gas chromatographic method for the determination of nifedipine in human plasma is presented. Nifedipine was extracted into toluene and diazepam was used as the internal standard. Chromatography was performed on an OV-101 fused-silica column by splitless injection with an electron capture detector. The minimal detectable concentration in plasma was 1 ng.ml-1. The standard curve was linear in the range 2-150 ng.ml-1 (gamma = 0.9988). The method has been applied to pharmacokinetic study.
介绍了一种测定人血浆中硝苯地平的气相色谱法。硝苯地平被萃取到甲苯中，地西泮用作内标。采用不分流进样，在OV - 101熔融石英柱上进行色谱分析，使用电子捕获检测器。血浆中的最低检测浓度为1 ng·ml⁻¹。标准曲线在2 - 150 ng·ml⁻¹范围内呈线性（γ = 0.9988）。该方法已应用于药代动力学研究。
