使用高效液相色谱法同时测定尿液中的烟酸及其主要代谢产物烟尿酸：固液萃取的应用

Simultaneous measurement of nicotinic acid and its major metabolite, nicotinuric acid in urine using high-performance liquid chromatography: application of solid-liquid extraction.

作者信息

Iwaki M, Ogiso T, Hayashi H, Lin E T, Benet L Z

机构信息

Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.

出版信息

J Chromatogr B Biomed Appl. 1994 Nov 4;661(1):154-8. doi: 10.1016/0378-4347(94)00332-7.

DOI:10.1016/0378-4347(94)00332-7

PMID:7866544

Abstract

The concentrations of nicotinic acid (NiAc) and nicotinuric acid (NiUAc), a major metabolite of NiAc, were simultaneously determined in urine using solid-phase extraction (cation-exchange extraction) and reversed-phase high-performance liquid chromatography with ultraviolet detection. The intra- and inter-day precision studies showed good reproducibilities: the coefficients of variations were less than 8.1% for NiAc and 8.8% for NiUAc. The calibration curves were linear (r2 > 0.9934) in the concentration range 10-1000 micrograms/ml. The removal of endogenous interferences in urine by solid-phase extraction presented here is superior to the pretreatment protocols reported previously by other workers. The method was used in a preliminary pharmacokinetic study in rats after intravenous administration of NiAc (5 and 15 mg/kg).

摘要

采用固相萃取（阳离子交换萃取）和带紫外检测的反相高效液相色谱法，同时测定尿液中烟酸（NiAc）及其主要代谢物烟尿酸（NiUAc）的浓度。日内和日间精密度研究显示出良好的重现性：NiAc的变异系数小于8.1%，NiUAc的变异系数小于8.8%。在10 - 1000微克/毫升的浓度范围内，校准曲线呈线性（r2 > 0.9934）。本文介绍的固相萃取法去除尿液中内源性干扰的效果优于其他研究人员先前报道的预处理方案。该方法用于大鼠静脉注射NiAc（5和15毫克/千克）后的初步药代动力学研究。