Miyauchi Y, Sano N, Nakamura T
Eisai Research Laboratories, Eisai Co., Ltd., Tokyo, Japan.
Int J Vitam Nutr Res. 1993;63(2):145-9.
A high performance liquid chromatographic method for the simultaneous quantitative determination of nicotinic acid, nicotinamide and nicotinuric acid in human plasma is described. The method is based on solid phase extraction in combination with ion-paired reversed phase high performance liquid chromatography. Recoveries of nicotinic acid, nicotinamide and nicotinuric acid are 92.9, 95.8 and 87.8%, respectively. The procedure shown was applied to the determination of the plasma time-concentration profile of nicotinic acid, nicotinamide and nictotinuric acid after nicotinic acid ingestion in humans.
本文描述了一种同时定量测定人血浆中烟酸、烟酰胺和烟尿酸的高效液相色谱方法。该方法基于固相萃取结合离子对反相高效液相色谱。烟酸、烟酰胺和烟尿酸的回收率分别为92.9%、95.8%和87.8%。所示方法应用于测定人体摄入烟酸后血浆中烟酸、烟酰胺和烟尿酸的时间-浓度曲线。
