Sasaki Y, Shiba Y, Kanno Y
Department of Oral Physiology, Hiroshima University School of Dentistry, Japan.
Comp Biochem Physiol A Physiol. 1995 Jan;110(1):57-64. doi: 10.1016/0300-9629(94)00148-m.
In this study, the effects of parasympathectomy and cholinergic agonists on gap-junctional intercellular communication and salivary secretion were investigated to clarify the involvement of salivary secretion in delayed uncoupling between acinar cells of rat submandibular glands. Gap-junctional intercellular communication was monitored as dye-coupling in the acinar cells of isolated acini by the transfer of Lucifer Yellow CH. Parasympathectomy induced dye-uncoupling in the acinar cells isolated from denervated salivary glands 12 hr after parasympathectomy-induced salivary secretion. Intraperitoneal application of carbachol (CCh), acetylcholine, pilocarpine, but not isoproterenol, stimulated salivary secretion, and then induced dye-uncoupling in the acinar cells 12 hr later. Atropine suppressed both the salivary secretion and delayed dye-uncoupling induced by parasympathectomy and CCh, when atropine was applied intraperitoneally before the induction of salivary secretion. However, atropine did not suppress the delayed dye-uncoupling by intraperitoneal application of CCh, when atropine was injected after the cessation of CCh-induced secretion. These results suggest that delayed inhibition of gap-junctional intercellular communication by parasympathectomy and cholinergic agonists in rat submandibular glands might be related to the change of secretory function after salivary secretion.
在本研究中，研究了去交感神经支配和胆碱能激动剂对缝隙连接细胞间通讯及唾液分泌的影响，以阐明唾液分泌在大鼠下颌下腺腺泡细胞延迟解偶联中的作用。通过 Lucifer Yellow CH 的转移，将缝隙连接细胞间通讯监测为分离腺泡的腺泡细胞中的染料偶联。去交感神经支配在诱导唾液分泌 12 小时后，可诱导去神经支配唾液腺分离出的腺泡细胞发生染料解偶联。腹腔注射卡巴胆碱（CCh）、乙酰胆碱、毛果芸香碱，但不包括异丙肾上腺素，可刺激唾液分泌，并在 12 小时后诱导腺泡细胞发生染料解偶联。当在诱导唾液分泌前腹腔注射阿托品时，阿托品可抑制去交感神经支配和 CCh 诱导的唾液分泌及延迟染料解偶联。然而，当在 CCh 诱导的分泌停止后注射阿托品时，阿托品并不能抑制腹腔注射 CCh 引起的延迟染料解偶联。这些结果表明，去交感神经支配和胆碱能激动剂对大鼠下颌下腺缝隙连接细胞间通讯的延迟抑制可能与唾液分泌后分泌功能的变化有关。
