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凝集素亲和色谱法

Lectin affinity chromatography.

作者信息

West I, Goldring O

出版信息

Mol Biotechnol. 1994 Oct;2(2):147-55. doi: 10.1007/BF02824807.

DOI:10.1007/BF02824807
PMID:7866873
Abstract

A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyldiimidazole-activated agarose are described.

摘要

本文描述了一种使用纯化凝集素和预活化基质制备凝集素亲和色谱柱的方案。给出了一种在固定化伴刀豆球蛋白A上纯化糖蛋白的通用方法。描述了将伴刀豆球蛋白A固定在碳二亚胺琼脂糖、Affi-Gel 15和羰基二咪唑活化琼脂糖上的方法。

相似文献

1
Lectin affinity chromatography.凝集素亲和色谱法
Mol Biotechnol. 1994 Oct;2(2):147-55. doi: 10.1007/BF02824807.
2
Lectin affinity chromatography.凝集素亲和色谱法。
Curr Protoc Protein Sci. 2001 May;Chapter 9:Unit 9.1. doi: 10.1002/0471140864.ps0901s00.
3
Activities of lectins and their immobilized derivatives in detergent solutions. Implications on the use of lectin affinity chromatography for the purification of membrane glycoproteins.凝集素及其固定化衍生物在洗涤剂溶液中的活性。对使用凝集素亲和色谱法纯化膜糖蛋白的启示。
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Isolation of equine infectious anemia virus glycoproteins. Lectin affinity chromatography procedures for high avidity glycoproteins.马传染性贫血病毒糖蛋白的分离。用于高亲和力糖蛋白的凝集素亲和层析程序。
J Virol Methods. 1983 Jun;6(6):337-46. doi: 10.1016/0166-0934(83)90056-3.
5
Purification of the glycoprotein allergen Ag7 from mugwort pollen by concanavalin A affinity chromatography.用伴刀豆球蛋白A亲和层析法从艾蒿花粉中纯化糖蛋白变应原Ag7。
J Biotechnol. 1990 Nov;16(3-4):305-16. doi: 10.1016/0168-1656(90)90044-c.
6
Lectin affinity chromatography.凝集素亲和层析法
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Affinity chromatography with monolithic capillary columns. II. Polymethacrylate monoliths with immobilized lectins for the separation of glycoconjugates by nano-liquid affinity chromatography.整体式毛细管柱亲和色谱法。II. 固定化凝集素的聚甲基丙烯酸酯整体柱用于纳米液相亲和色谱法分离糖缀合物
J Chromatogr A. 2005 Jun 24;1079(1-2):236-45. doi: 10.1016/j.chroma.2005.02.084.
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Affinity chromatography with agarose-lectin derivatives: separation of human glycoproteins and application to erythropoietin purification.琼脂糖-凝集素衍生物亲和层析：人糖蛋白的分离及其在促红细胞生成素纯化中的应用
Prog Clin Biol Res. 1979;29:641-53.
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Preparation of affinity adsorbents and purification of lectins from natural sources.亲和吸附剂的制备及天然来源凝集素的纯化。
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Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.人成纤维细胞干扰素与伴刀豆球蛋白A-琼脂糖的结合。碳水化合物识别和疏水相互作用的参与。
Biochemistry. 1976 Feb 10;15(3):704-13. doi: 10.1021/bi00648a039.

本文引用的文献

1
Schistosoma mansoni: surface membrane isolation with lectin-coated beads.曼氏血吸虫：用凝集素包被的珠子进行表面膜分离
Membr Biochem. 1993 Jul-Sep;10(3):155-61. doi: 10.3109/09687689309150262.
2
A simple and effective solvent system for elution of gonadotropins from concanavalin A affinity chromatography.一种用于从伴刀豆球蛋白A亲和色谱中洗脱促性腺激素的简单有效的溶剂系统。
Anal Biochem. 1980 Aug;106(2):402-10. doi: 10.1016/0003-2697(80)90540-0.
3
Isolation of the major glycoprotein from fat cell plasma membranes.
Arch Biochem Biophys. 1980 Jan;199(1):84-91. doi: 10.1016/0003-9861(80)90259-3.
4
Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins.十二种凝集素对与N-糖基化蛋白相关的寡糖和糖肽的特异性。
Eur J Biochem. 1981 Jun;117(1):41-55. doi: 10.1111/j.1432-1033.1981.tb06300.x.
5
Fractionation of asparagine-linked oligosaccharides by serial lectin-Agarose affinity chromatography. A rapid, sensitive, and specific technique.通过连续凝集素-琼脂糖亲和色谱法分离天冬酰胺连接的寡糖。一种快速、灵敏且特异的技术。
J Biol Chem. 1982 Oct 10;257(19):11235-40.
6
Resolution of carbohydrates by lectin affinity chromatography.通过凝集素亲和色谱法分离碳水化合物。
Methods Enzymol. 1982;83:127-32. doi: 10.1016/0076-6879(82)83009-7.
7
Affinity chromatography of cells and cell membranes.细胞和细胞膜的亲和层析
J Chromatogr. 1980 Nov 7;184(4):471-99. doi: 10.1016/s0021-9673(00)93875-5.
8
Turnover and orientation of the major neural retina cell surface protein protected from tryptic cleavage by calcium.主要神经视网膜细胞表面蛋白的更新与取向，该蛋白受钙保护不被胰蛋白酶切割。
Biochemistry. 1984 Feb 28;23(5):899-904. doi: 10.1021/bi00300a016.
9
A method for efficient and selective recovery of membrane glycoproteins from concanavalin A-Sepharose using media containing sodium dodecyl sulfate and urea.
Anal Biochem. 1980 Dec;109(2):460-5. doi: 10.1016/0003-2697(80)90678-8.
10
Concanavalin A--Sepharose purification of soluble Na,K-ATPase from rectal glands of the spiny dogfish.伴刀豆球蛋白A-Sepharose从棘鲨直肠腺中纯化可溶性钠钾ATP酶
Anal Biochem. 1980 Oct;108(1):83-5. doi: 10.1016/0003-2697(80)90695-8.