凝集素亲和色谱法
Lectin affinity chromatography.
作者信息
West I, Goldring O
出版信息
Mol Biotechnol. 1994 Oct;2(2):147-55. doi: 10.1007/BF02824807.
PMID:7866873
Abstract
A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyldiimidazole-activated agarose are described.
摘要
本文描述了一种使用纯化凝集素和预活化基质制备凝集素亲和色谱柱的方案。给出了一种在固定化伴刀豆球蛋白A上纯化糖蛋白的通用方法。描述了将伴刀豆球蛋白A固定在碳二亚胺琼脂糖、Affi-Gel 15和羰基二咪唑活化琼脂糖上的方法。
Zotero 插件