Sharma S K, Mahendroo P P
J Chromatogr. 1980 Nov 7;184(4):471-99. doi: 10.1016/s0021-9673(00)93875-5.
This article attempts to introduce the concept of cellular affinity chromatography as it departs from molecular affinity chromatography. Special emphasis is placed on the selectio of a solid support as well as on the role of lectins as affinity ligands. Our major goal was to bring to light the basic principles involved, multiple options of ligands and matrices, and different techniques, which may be applied to separate the complex cell population as well as cell membranes. It is hoped that further developments in the field, especially in the selection of proper experimental conditions, ligands and matrix material may provide better results. We have tried to identify some of the potential problems which should be considered before these approaches can be used on a routine basis. Although the review deals primarily with the affinity chromatography of cells and cell membranes, examples are presented for diverse systems such as cell organelles, viruses and phages. A table summarizing the use of cellular affinity chromatography is included. It lists more than 80 examples covering the literature up to December, 1979.
本文试图介绍细胞亲和色谱法这一概念，因为它与分子亲和色谱法有所不同。特别强调了固体支持物的选择以及凝集素作为亲和配体的作用。我们的主要目标是揭示其中涉及的基本原理、配体和基质的多种选择以及不同的技术，这些技术可用于分离复杂的细胞群体以及细胞膜。希望该领域的进一步发展，尤其是在选择合适的实验条件、配体和基质材料方面，能带来更好的结果。我们试图找出在这些方法能够常规使用之前应考虑的一些潜在问题。尽管该综述主要涉及细胞和细胞膜的亲和色谱法，但也给出了各种系统的实例，如细胞器、病毒和噬菌体。文中还包含一个总结细胞亲和色谱法应用的表格。它列出了截至1979年12月的80多个文献实例。
