Li W, Ohlmeyer J T, Lane M E, Kalderon D
Department of Biological Sciences, Columbia University, New York, New York 10027.
Cell. 1995 Feb 24;80(4):553-62. doi: 10.1016/0092-8674(95)90509-x.
Reduced protein kinase A (PKA) activity in anterior imaginal disc cells leads to cell-autonomous induction of decapentaplegic (dpp), wingless (wg), and patched (ptc) transcription that is independent of hedgehog (hh) gene activity. The resulting nonautonomous adult wing and leg pattern duplications are largely due to induced dpp and wg expression and resemble phenotypes elicited by ectopic hh expression. Inhibition of PKA in anterior cells close to the posterior compartment can substitute for hh activity to promote growth of imaginal discs, whereas overexpression of PKA can counteract transcriptional induction of ptc by hh in these cells. PKA therefore appears to be an integral component of the mechanism by which hh regulates the expression of key patterning molecules in imaginal discs.
前成虫盘细胞中蛋白激酶A（PKA）活性降低会导致细胞自主诱导脱磷酸化（dpp）、无翅（wg）和patched（ptc）转录，这与刺猬基因（hh）的活性无关。由此产生的非自主成虫翅膀和腿部模式重复主要是由于诱导的dpp和wg表达，并且类似于由异位hh表达引发的表型。在前部靠近后部隔室的细胞中抑制PKA可以替代hh活性以促进成虫盘的生长，而PKA的过表达可以抵消hh在这些细胞中对ptc的转录诱导。因此，PKA似乎是hh调节成虫盘中关键模式分子表达机制的一个组成部分。
