Thompson J E, Phillips R J, Erdjument-Bromage H, Tempst P, Ghosh S
Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520.
Cell. 1995 Feb 24;80(4):573-82. doi: 10.1016/0092-8674(95)90511-1.
We have cloned the cDNA encoding I kappa B-beta, one of the two major I kappa B isoforms in mammalian cells. The recombinant I kappa B- beta protein interacts with equal affinity to p65 and c-Rel and does not exhibit a preference between these Rel proteins. Instead the primary difference between I kapp B-alpha and I kappa B-beta is in their response to different inducers of NF-kappa B activity. One class of inducers causes rapid but transient activation of NF-kappa B by primarily affecting I kappa B-alpha complexes, whereas another class of inducers causes persistent activation of NF-kapa B by affecting both I kappa B-alpha and I kappa B-beta complexes. Therefore, the overall activation of NF-kappa B consists of two overlapping phases, a transient phase mediated through I kappa B-alpha and a persistent phase mediated through I kappa B-beta.
我们克隆了编码IκB-β的cDNA，IκB-β是哺乳动物细胞中两种主要的IκB亚型之一。重组IκB-β蛋白与p65和c-Rel具有相同的亲和力，并且在这些Rel蛋白之间没有表现出偏好。相反，IκB-α和IκB-β之间的主要差异在于它们对NF-κB活性不同诱导剂的反应。一类诱导剂主要通过影响IκB-α复合物导致NF-κB快速但短暂的激活，而另一类诱导剂通过影响IκB-α和IκB-β复合物导致NF-κB持续激活。因此，NF-κB的整体激活包括两个重叠阶段，一个通过IκB-α介导的短暂阶段和一个通过IκB-β介导的持续阶段。
