Weih F, Carrasco D, Bravo R
Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.
Oncogene. 1994 Nov;9(11):3289-97.
We have studied the expression of members of the rel family of transcription factors and ikba in mouse thymus and spleen by in situ hybridization. Our results show that the rel genes have different temporal and spatial patterns of expression suggesting distinct roles in these lymphoid tissues. The Rel/NF-kappa B proteins and I kappa B alpha in thymus and spleen were also analysed by Western blotting and electrophoretic mobility shift assays. Although RelB protein is present at significantly lower levels in thymus and spleen extracts when compared to RelA, in both tissues the predominant kappa B-binding activity consists of p50/RelB and p52/RelB heterodimers and only very little binding of RelA-containing complexes to kappa B sites was detected. Significant binding of c-Rel complexes was only found in spleen extracts. Treatment of thymus and spleen extracts with deoxycholate (DOC), however, results in a strong increase in binding to kappa B sites of both RelA and c-Rel complexes. In contrast, binding of RelB complexes is not induced after DOC treatment. Our results suggest a differential role of Rel/NF-kappa B complexes in mouse thymus and spleen with RelB heterodimers representing the constitutive kappa B-binding activity, whereas RelA and c-Rel complexes most likely are involved in inducible kappa B-binding and gene activation.
我们通过原位杂交研究了转录因子rel家族成员和IκBα在小鼠胸腺和脾脏中的表达。我们的结果表明，rel基因具有不同的时空表达模式，提示它们在这些淋巴组织中发挥着不同的作用。还通过蛋白质免疫印迹法和电泳迁移率变动分析对胸腺和脾脏中的Rel/NF-κB蛋白及IκBα进行了分析。尽管与RelA相比，RelB蛋白在胸腺和脾脏提取物中的含量显著较低，但在这两种组织中，主要的κB结合活性由p50/RelB和p52/RelB异二聚体组成，仅检测到极少量含RelA的复合物与κB位点的结合。仅在脾脏提取物中发现了c-Rel复合物的显著结合。然而，用脱氧胆酸盐（DOC）处理胸腺和脾脏提取物后，RelA和c-Rel复合物与κB位点的结合显著增加。相比之下，DOC处理后未诱导RelB复合物的结合。我们的结果提示Rel/NF-κB复合物在小鼠胸腺和脾脏中具有不同的作用，其中RelB异二聚体代表组成型κB结合活性，而RelA和c-Rel复合物很可能参与诱导性κB结合和基因激活。
