Yang X, Reng L, Ding F
Cancer Institute, CAMS and PUMC, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1994 Aug;16(4):245-50.
As previously reported, transfer of RA538 into parental esophageal cancer EC8712 cell line induced its terminal differentiation and apoptosis. To further study the biological effects of this cDNA, an expression plasmid containing an insert of the putative coding fragment (about 0.3kb) of RA538 and neo resistance gene was constructed (designated pRA538-0.3-neo) and transferred into three different human cancer cell lines: EC8712, HL60 and GLC, a cell line derived from an adenocarcinoma of the lung. After selection in G418-containing culture media, the growth rate, 3H-thymidine incorporation rate, cell morphology, colony-formation in soft-agar, and heterotransplantation into nude mice of the surviving cell populations were tested. In situ hybridization verified the uptake and expression of the 0.3kb fragment of RA538 in these G418 resistant cell populations. Significant reduction in growth rate and suppression of malignant phenotype were observed in all these cells in comparison with their parental cancer cell lines.
如先前报道，将RA538导入亲代食管癌EC8712细胞系可诱导其终末分化和凋亡。为进一步研究该cDNA的生物学效应，构建了一个表达质粒，其插入了RA538假定编码片段（约0.3kb）和新霉素抗性基因（命名为pRA538-0.3-neo），并将其导入三种不同的人类癌细胞系：EC8712、HL60和GLC（一种源自肺腺癌的细胞系）。在含G418的培养基中筛选后，检测存活细胞群体的生长速率、3H-胸腺嘧啶核苷掺入率、细胞形态、软琼脂中的集落形成以及裸鼠体内异种移植情况。原位杂交证实了RA538的0.3kb片段在这些对G418有抗性的细胞群体中的摄取和表达。与它们的亲代癌细胞系相比，在所有这些细胞中均观察到生长速率显著降低和恶性表型受到抑制。
