Gröhn P, Klöck G, Schmitt J, Zimmermann U, Horcher A, Bretzel R G, Hering B J, Brandhorst D, Brandhorst H, Zekorn T
Lehrstuhl für Biotechnologie, Universität, Biozentrum, Würzburg, Germany.
Exp Clin Endocrinol. 1994;102(5):380-7. doi: 10.1055/s-0029-1211308.
Islet xenografts immunisolated in alginate capsules have been proposed by many groups for clinical islet transplantation. However, diffusion limitations and the total volume of microcapsules required for transplantation are, among other things, factors which have so far prevented successful clinical application. In this study, these problems have been overcome by immobilisation of rat and porcine islets in a Ba(2+)-cross-linked alignate matrix using an air jet droplet generator technique in combination with subsequent density-gradient purification. This procedure leads to high yields of islets coated with a tailored, thin layer of cross-linked alginate which protects the islets against cytotoxic compounds present in human plasma. The recovery of encapsulated rat and porcine islets was about 70%. Empty capsules were nearly completely removed. Using this technique, the total volume of the resulting microcapsules increased only by a factor of about 1.5 compared to the volume of free islets. The technique can be used for large-scale production of coated islets.
许多研究团队都提出将免疫隔离在藻酸盐胶囊中的胰岛用于临床胰岛移植。然而，扩散限制以及移植所需微胶囊的总体积等因素，是迄今为止阻碍其成功临床应用的部分原因。在本研究中，通过使用空气喷射液滴发生器技术并结合后续的密度梯度纯化，将大鼠和猪的胰岛固定在Ba(2+)交联的藻酸盐基质中，克服了这些问题。该方法可使胰岛获得高产率，且表面包裹着一层定制的、薄的交联藻酸盐层，可保护胰岛免受人血浆中存在的细胞毒性化合物的影响。封装的大鼠和猪胰岛回收率约为70%。空胶囊几乎被完全去除。使用该技术，所得微胶囊的总体积仅比游离胰岛的体积增加了约1.5倍。该技术可用于大规模生产包被胰岛。
