Rush C, Willetts A, Davies G, Dauter Z, Watson H, Littlechild J
Department of Chemistry, University of Exeter, UK.
FEBS Lett. 1995 Feb 13;359(2-3):244-6. doi: 10.1016/0014-5793(95)00055-e.
The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised. The protein is reported to be a hexamer of 12 x 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride. They are stable and diffract to better than 2 A resolution. They are of a cubic space group I23 (or 12(1)3) with cell dimensions a = b = c = 310 A.
