Scripps Institution of Oceanography, University of California, San Diego, California 92093, USA.
J Am Chem Soc. 2011 Mar 30;133(12):4268-70. doi: 10.1021/ja201088k. Epub 2011 Mar 8.
Halogenases catalyze reactions that introduce halogen atoms into electron-rich organic molecules. Vanadium-dependent haloperoxidases are generally considered to be promiscuous halogenating enzymes that have thus far been derived exclusively from eukaryotes, where their cellular function is often disputed. We now report the first biochemical characterization of a bacterial vanadium-dependent chloroperoxidase, NapH1 from Streptomyces sp. CNQ-525, which catalyzes a highly stereoselective chlorination-cyclization reaction in napyradiomycin antibiotic biosynthesis. This finding biochemically links a vanadium chloroperoxidase to microbial natural product biosynthesis.
卤化酶催化将卤素原子引入富电子有机分子的反应。钒依赖的卤过氧化物酶通常被认为是混杂的卤化酶，迄今为止仅从真核生物中衍生而来，其细胞功能常常存在争议。我们现在报告了第一个细菌钒依赖的氯过氧化物酶的生化特征，即来自链霉菌属 CNQ-525 的 NapH1，它在萘啶抗生素生物合成中催化高度立体选择性的氯化环化反应。这一发现从生物化学上把一种钒氯过氧化物酶与微生物天然产物生物合成联系起来。
