Sailstad D M, Tepper J S, Doerfler D L, Qasim M, Selgrade M K
ManTech Environmental Technology, Inc., Research Triangle Park, North Carolina 27709.
Fundam Appl Toxicol. 1994 Nov;23(4):569-77. doi: 10.1006/faat.1994.1142.
Two dye mixtures and the individual component dyes were evaluated for the potential to induce contact or pulmonary hypersensitivity. These dye mixtures were suspect because of anecdotal reports of both pulmonary and contact hypersensitivity in assembly workers, and because the component dyes were structurally related to dyes known to be contact sensitizers. One mixture consisted of disperse blue 3 (DB3) and disperse red 11 (DR11), which are anthraquinones, and the other mixture contained DR11 and solvent red 1 (SR1), an azo dye. Contact hypersensitivity was examined using the local lymph node assay (LLNA) and a modified mouse ear swelling test (MEST). Both the MEST and the LLNA indicated that SR1 has weak contact-sensitizing potential. None of the other individual dye compounds or the two mixtures were identified as contact sensitizers by either method. To evaluate the mixtures as potential pulmonary allergens, guinea pigs were repeatedly exposed by inhalation (300 mg/m3, 6 hr/day) 5 days/week, for 1 week. Weekly exposures were repeated three times with 2 weeks of nonexposure time in between. Guinea pigs were then challenged through the jugular vein using a dye-dimethylsulfoxide mixture. During the challenge, breathing mechanics (dynamic compliance and resistance) were measured in mechanically ventilated animals. Changes in these measurements, indicative of bronchoconstriction, were not observed in animals exposed to either dye mixture, nor were antibodies detected in the sera of exposed animals using individual dye-specific enzyme-linked immunosorbent assays. In conclusion, two methods indicate that SR1 may have contact-sensitizing potential.(ABSTRACT TRUNCATED AT 250 WORDS)
对两种染料混合物及其各成分染料进行了评估，以确定其诱发接触性或肺部超敏反应的可能性。由于有装配工人出现肺部和接触性超敏反应的传闻报道，且这些成分染料在结构上与已知的接触致敏染料相关，所以这些染料混合物受到怀疑。一种混合物由蒽醌类的分散蓝3（DB3）和分散红11（DR11）组成，另一种混合物包含偶氮染料DR11和溶剂红1（SR1）。使用局部淋巴结试验（LLNA）和改良的小鼠耳肿胀试验（MEST）检测接触性超敏反应。MEST和LLNA均表明SR1具有较弱的接触致敏潜力。两种方法均未将其他单一染料化合物或这两种混合物鉴定为接触致敏剂。为了评估这些混合物作为潜在肺部过敏原的可能性，豚鼠每周5天、每天6小时反复吸入暴露（300 mg/m³），持续1周。每周暴露重复3次，中间间隔2周不暴露。然后使用染料 - 二甲基亚砜混合物通过颈静脉对豚鼠进行激发试验。在激发试验期间，对机械通气的动物测量呼吸力学（动态顺应性和阻力）。在暴露于任一种染料混合物的动物中均未观察到这些测量值的变化（表明支气管收缩），使用针对单一染料的酶联免疫吸附测定法在暴露动物的血清中也未检测到抗体。总之，两种方法表明SR1可能具有接触致敏潜力。（摘要截断于250字）
